Illumina MiSeq sequencing; qiita_ptid_2362:10317.000059083 - SRA

ERX1904881: Illumina MiSeq sequencing; qiita_ptid_2362:10317.000059083
1 ILLUMINA (Illumina MiSeq) run: 20,228 spots, 3M bases, 2.2Mb downloads

Design: fecal, saliva, skin and environment samples from the American Gut Project

Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)

Study: American Gut Project

PRJEB11419 • ERP012803 • All experiments • All runs

show Abstracthide Abstract

The American Gut project is the largest crowdsourced citizen science project to date. Fecal, oral, skin, and other body site samples collected from thousands of participants represent the largest human microbiome cohort in existence. Detailed health and lifestyle and diet data associated with each sample is enabling us to deeply examine associations between the human microbiome and factors such as diet (from vegan to near carnivore and everything in between), season, amount of sleep, and disease states such as IBD, diabetes, or autism spectrum disorder-as well as many other factors not listed here. The American Gut project also encompasses the British Gut and Australian Gut projects, widening the cohort beyond North America. As the project continues to grow, we will be able to identify significant associations that would not be possible with smaller, geographically and health/disease status-limited cohorts.

Sample: qiita_sid_10317:10317.000059083

SAMEA93260668 • ERS1562462 • All experiments • All runs

Organism: human gut metagenome

Library:

Name: 10317.000059083

Instrument: Illumina MiSeq

Strategy: OTHER

Source: METAGENOMIC

Selection: PCR

Layout: SINGLE

Construction protocol: Illumina MiSeq 515fbc, 806r amplification of 16SrRNA V4

Experiment attributes: (show all 31 attributes...) (hide...)

barcode: CGGCGATGAAAG

center_name: UCSDMI

center_project_name: AG33

experiment_center: UCSDMI

experiment_title: American Gut Project

extraction_robot: HOWE_KF1

extractionkit_lot: PM16G13

linker: GT

mastermix_lot: 396036

orig_name: 59083

pcr_primers: FWD:GTGYCAGCMGCCGCGGTAA; REV:GGACTACNVGGGTWTCTAAT

plating: LDG

primer: GTGTGYCAGCMGCCGCGGTAA

primer_date: 110716

primer_plate: 7

processing_robot: CARMEN

project_name: American_Gut_Project

run_center: UCSDMI

run_date: 12/05/16

run_prefix: RQ_3D_Mouse_2

samp_size: 0.1,g

sample_plate: American_Gut_Project_Plate_147

sequencing_meth: Sequencing by synthesis

target_gene: 16S rRNA

target_subfragment: V4

tm1000_8_tool: 108379Z

tm300_8_tool: 3076189

tm50_8_tool: 409172Z

water_lot: RNBF2734

well_description: American_Gut_Project_Plate_147_59083_F4

well_id: F4

Runs: 1 run, 20,228 spots, 3M bases, 2.2Mb

Run	# of Spots	# of Bases	Size	Published
ERR1842904	20,228	3M	2.2Mb	2017-02-21

ID:: 3736587

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